HART may refer to:
- Halt All Racist Tours, New Zealand
 - Health Advisory and Recovery Team, a British antivaccine pressure group
 - Heterogeneous Aerial Reconnaissance Team
 - Highway Addressable Remote Transducer Protocol
 - Hillsboro Artists' Regional Theatre
 - Human Assisted Reproductive Technology
 - Hypervelocity Aircraft Rocket, Tactical
 
Transportation
- Hillsborough Area Regional Transit, Florida
 - Honolulu Authority for Rapid Transportation, Hawaiʻi
 - Housatonic Area Regional Transit, Connecticut
 - Huntington Area Rapid Transit, Long Island, New York
 
Response or rescue teams
- Hazardous area response team, UK
 - High Acuity Response Team, a type of Rapid response team (medicine)
 
Science and technology
- HART Communications Protocol (Highway Addressable Remote Transducer) - a communication protocol used in the process industries.
 
See also
- Highly active antiretroviral therapy (HAART), a treatment for HIV
 
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