MFI or M.F.I. may refer to:
Companies and organizations
- MFI Foundation Inc., non-profit science foundation in the Philippines
 - MFI Group, British furniture retailer
 - Media Factory, Inc., anime company
 - Messiah Foundation International, a spiritual organization
 - Microfinance institution, alternate form of bank in developing countries which provides microcredit lending
 - MindFreedom International, group that fights involuntary therapy
 - Ministers Fellowship International, fellowship of non-denominational charismatic churches
 - Missionary Flights International, a religious charity providing aviation support with DC-3 aircraft
 
Other
- MFi Program, "Made for iPhone/iPod/iPad", a licensing program for developers of hardware and software for Apple products
 - Magnetic field imaging, a noninvasive and contact-free cardiac diagnostic method
 - Melt flow index, a measure of the ease of flow of the melt of a thermoplastic polymer
 - Micromechanical Flying Insect, ornithopter robot that flies based on insect flight technique
 - Mikoyan Project 1.44, MiG-MFI, Russian fighter aircraft project (cancelled)
 - Minimum Fisher information, variational principle in information theory
 - Money flow index, indicator in technical analysis
 
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