The 2016 Copa do Brasil Second Round will be played from 3 May to 6 July 2016, to decide the 20 teams advancing to the Third Round.[1]
Matches
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Santos  | 
3–0 | 3–0 | – | |
| ABC  | 
2–2 (1–4 p) | 1–1 | 1–1 | |
| Botafogo  | 
2–0 | 1–0 | 1–0 | |
| Ceará  | 
2–0 | 1–0 | 1–0 | |
| Vasco da Gama  | 
2–1 | 1–0 | 1–1 | |
| Santa Cruz  | 
2–0 | 2–0 | – | |
| Cruzeiro  | 
2–0 | 2–0 | – | |
| Vitória  | 
3–1 | 0–0 | 3–1 | |
| Coritiba  | 
2–3 | 0–1 | 2–2 | |
| Paysandu  | 
2–1 | 0–1 | 2–0 | |
| Atlético Paranaense  | 
7–2 | 2–2 | 5–0 | |
| Chapecoense  | 
3–2 | 1–2 | 2–0 | |
| Flamengo  | 
2–4 | 1–2 | 1–2 | |
| Bahia  | 
0–1 | 0–0 | 0–1 | |
| Fluminense  | 
6–3 | 3–3 | 3–0 | |
| Aparecidense  | 
3–4 | 1–3 | 2–1 | |
| Figueirense  | 
3–1 | 2–1 | 1–0 | |
| Ponte Preta  | 
4–0 | 1–0 | 3–0 | |
| Botafogo  | 
3–1 | 2–1 | 1–0 | |
| Avaí  | 
1–2 | 0–1 | 1–1 | 
Match 41
| Galvez  | 0–3 | |
|---|---|---|
| Report | Rafael Longuine  Paulinho Fernando Medeiros  | 
Santos advanced directly due to winning by 2 or more goals difference.
Match 42
| Gama  | 1–1 | |
|---|---|---|
| Roberto Pítio  | 
Report | Nando  | 
| ABC  | 1–1 | |
|---|---|---|
| Erivélton  | 
Report | Felipe Assis  | 
| Penalties | ||
| Jones Carioca  Felipe Guedes Victor Sapo  | 
1–4 | Murilo  Roberto Pítio Baiano Fábio Gama  | 
Tied 2–2 on aggregate, Gama won on penalties.
Match 43
Botafogo won 2–0 on aggregate
Match 44
| Joinville  | 0–1 | |
|---|---|---|
| Report | Rafael Costa  | 
| Ceará  | 1–0 | |
|---|---|---|
| Tomas Bastos  | 
Report | 
Ceará won 2–0 on aggregate.
Match 45
| CRB  | 0–1 | |
|---|---|---|
| Report | Rodrigo  | 
| Vasco da Gama  | 1–1 | |
|---|---|---|
| Rafael Vaz  | 
Report | Diego  | 
Vasco da Gama won 2–1 on aggregate.
Match 46
| Vitória da Conquista  | 0–2 | |
|---|---|---|
| Report | Bruno Moraes  | 
Santa Cruz advanced directly due to winning by 2 or more goals difference.
Match 47
| Londrina  | 0–2 | |
|---|---|---|
| Report | Bruno Rodrigo  Henrique  | 
Cruzeiro advanced directly due to winning by 2 or more goals difference.
Match 48
| Portuguesa  | 0–0 | |
|---|---|---|
| Report | 
| Vitória  | 3–1 | |
|---|---|---|
| Diego Renan  Marcelo Kieza  | 
Report | Diego Gonçalves  | 
Vitória won 3–1 on aggregate.
Match 49
Juventude won 3–2 on aggregate.
Match 50
| Operário  | 1–0 | |
|---|---|---|
| Washington  | 
Report | 
Paysandu won 2–1 on aggregate.
Match 51
| Dom Bosco  | 2–2 | |
|---|---|---|
| Naian  Fernando Nathan  | 
Report | Vinícius  André Lima  | 
| Atlético Paranaense  | 5–0 | |
|---|---|---|
| André Lima  Pablo Hernani Anderson Lopes  | 
Report | 
Atlético Paranaense won 7–2 on aggregate.
Match 52
| Paraná  | 2–1 | |
|---|---|---|
| Nádson  | 
Report | Bruno Rangel  | 
| Chapecoense  | 2–0 | |
|---|---|---|
| Kempes  Cléber Santana  | 
Report | 
Chapecoense won 3–2 on aggregate.
Match 53
| Flamengo  | 1–2 | |
|---|---|---|
| Alan Patrick  | 
Report | Pio  | 
Fortaleza won 4–2 on aggregate.
Match 54
| América Mineiro  | 0–0 | |
|---|---|---|
| Report | 
| Bahia  | 0–1 | |
|---|---|---|
| Report | Suéliton  | 
América Mineiro won 1–0 on aggregate.
Match 55
| Ferroviária  | 3–3 | |
|---|---|---|
| Luan  Tiago Marques  | 
Report | Fred  Magno Alves  | 
| Fluminense  | 3–0 | |
|---|---|---|
| Gustavo Scarpa  Fred  | 
Report | 
Fluminense won 6–3 on aggregate.
Match 56
| Ypiranga  | 3–1 | |
|---|---|---|
| João Paulo  Danilinho  | 
Report | Clayton Sales  | 
| Aparecidense  | 2–1 | |
|---|---|---|
| Foguinho  Geovane  | 
Report | Maycon  | 
Ypiranga won 4–3 on aggregate.
Match 57
| Sampaio Corrêa  | 1–2 | |
|---|---|---|
| Edgar  | 
Report | Gustavo Ermel  Guilherme Queiróz  | 
| Figueirense  | 1–0 | |
|---|---|---|
| Éverton Santos  | 
Report | 
Figueirense won 3–1 on aggregate.
Match 58
| Genus  | 0–1 | |
|---|---|---|
| Report | Felipe Azevedo  | 
| Ponte Preta  | 3–0 | |
|---|---|---|
| Matheus Jesus  Léo Cereja Wellington Paulista  | 
Report | 
Ponte Preta won 4–0 on aggregate.
Match 59
| Juazeirense  | 1–2 | |
|---|---|---|
| Alex Travassos  | 
Report | Neílton  Emerson  | 
| Botafogo  | 1–0 | |
|---|---|---|
| Neílton  | 
Report | 
Botafogo won 3–1 on aggregate.
Match 60
| Bragantino  | 1–0 | |
|---|---|---|
| Leandro Oliveira  | 
Report | 
| Avaí  | 1–1 | |
|---|---|---|
| Tauã  | 
Report | Zambi  | 
Bragantino won 2–1 on aggregate.